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regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1, Pages 87-96 doi: 10.15302/J-FASE-2016087

Abstract: that the pMAR-CAG-FST-IRES-AcGFP1-polyA-MAR (pMAR-FST) vector had higher capacity to form monoclonal transgenicRemarkably, protein expression in transgenic cells with the pMAR-FST vector was significantly higherExogenous was expressed in all of the pMAR-FST transgenic mice at F , F and F .mice was significantly greater than in wild-type mice, with larger muscles in fore and hind limbs of transgenicmice. pMAR-FST transgenic mice were also found with more evenly distributed muscle bundles and thinner

Keywords： safety of transgenic bicistron gene transfer body transgenic mice muscle development

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Functional characterization of caffeic acid

Fengyan WU, Zhenying WU, Aiguo YANG, Shanshan JIANG, Zeng-Yu WANG, Chunxiang FU

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 1, Pages 98-107 doi: 10.15302/J-FASE-2017198

Abstract: Transgenic switchgrass plants generated by an RNAi approach were further employed to study the functioncoupled with an obvious incorporation in 5-OH guaiacyl lignin units were observed in the COMT-RNAi transgenicbiochemical characterization of PvCOMT1, a significant decrease in sinapaldehyde was found in the COMT-RNAi transgenic

Keywords： biofuel crop caffeic acid O-methyltransferase forage lignin Panicum virgatum switchgrass transgenicplant

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Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Strategic Study of CAE 2002, Volume 4, Issue 8, Pages 34-39

Abstract: It described advances of transgenic breeding of rapeseed (Brassica napus) in Oil Crops Institute, HunanThe. paper dealt with Bt-transgenic insect-resistant rapeseed line, transgenic sterile line, restorerline and their hybrid, transgenic high oleic acid content rapeseed line and transgenic high oil content

Keywords： rapeseed transgenic breeding

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Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Strategic Study of CAE 2016, Volume 18, Issue 3, Pages 105-109 doi: 10.15302/J-SSCAE-2016.03.018

Abstract: the US Food and Drug Administration (FDA) approved the "all fish" growth hormone (gh) transgenicgenetically engineered livestock approved for human consumption and is considered as breakthrough of transgenicThe US FDA made this landmark decision after 20 years rigorous bio-safety assessment of the transgenicBoth the scientific research behind the creation of this transgenic product and the active participation

Keywords： transgenic Atlantic salmon industrialization enlightenments

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Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 1, Pages 66-72 doi: 10.15302/J-FASE-2018229

Abstract: transfected into porcine fetal fibroblasts, G418 resistant transfectants were confirmed by PCR and transgenicThese results have paved the way for future production of marker-free transgenic resistant to PEDV and

Keywords： piglet diarrhea RNAi PEDV TGEV transgenic resistance breeding

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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3, Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: One potential method is to produce transgenic livestock that make modified milk containing high concentrationSubsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generated transgenicanalysis showed that recombinant hBCHE (rhBCHE) was expressed efficiently in the mammary gland of the transgenicOur results pave the way for making transgenic livestock to produce large quantities of rhBCHE.

Keywords： recombinant human butyrylcholinesterase (rhBCHE) human lactoferrin bacterial artificial chromosome (hLF BAC) transgenic

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IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 4, Pages 635-644 doi: 10.15302/J-FASE -2020343

Abstract:

The antifungal effectiveness of extracts of five medicinal plant species was determined.chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plantAll plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v),Different extracts from the same plant species gave different degrees of inhibition.

Keywords： Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis

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ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 4, Pages 635-644 doi: 10.15302/J-FASE-2020343

Abstract: The antifungal effectiveness of extracts of five medicinal plant species was determined.inhibitory activity of extracts of , , , and were evaluated against the mycelial growth of the plantAll plant extracts were prepared at 60°C using solvents (either water, 50% ethanol ( ), 95% ethanol (Different extracts from the same plant species gave different degrees of inhibition.

Keywords： Botrytis cinerea food preservation plant diseases plant pathogenic fungi Tupistra chinensis

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conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 2, Pages 158-173 doi: 10.15302/J-FASE-2014015

Abstract: Bovine IgM was highly expressed in selected transgenic lines, and it largely inhibited rearrangementsAlthough the serum concentrations of endogenous IgM and IgG in the transgenic mice were significantlyThese transgenic mice also exhibited a lower frequency of unique complementarity determining region 3Compared to the WT mice, the transgenic mice had a significantly higher percentage of mouse IgM-expressingFinally, we showed that the transgenic mice were deficient in a specific antibody response to antigen

Keywords： bovine Ig μ heavy-chain transgenic mice B cell development allelic exclusion immune response Ig repertoire

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An introduction to the medicinal plant genome project

Shilin Chen, Li Xiang, Xu Guo, Qiushi Li

Frontiers of Medicine 2011, Volume 5, Issue 2, Pages 178-184 doi: 10.1007/s11684-011-0131-0

Abstract: In recent years, genomics has developed rapidly with the application of next-generation sequencing technology. However, very few studies have been carried out on genomics for medicinal plants. This paper introduces the genome research of medicinal plants, including genome sequencing, assembly, annotation, and functional genomics, to set up the foundation for the development of natural medicines and the selection of cultivars with good agricultural traits. This study places the study on traditional Chinese medicine into the frontier field of life science.

Keywords： genomics medicinal plant sequencing application

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HEMIPTERAN-TRANSMITTED PLANT VIRUSES: RESEARCH PROGRESS AND CONTROL STRATEGIES

Frontiers of Agricultural Science and Engineering 2022, Volume 9, Issue 1, Pages 98-109 doi: 10.15302/J-FASE-2021389

Abstract:

About 80% of plant viruses are transmitted by specific insect vectors, especially hemipterans withThis review summarizes the latest research findings on the interactions between plant viruses and insectvectors and analyzes the key factors affecting insect transmission of plant viruses from the perspectivestransmission of animal viruses, potential control strategies to prevent the transmission of insect-vectored plant

Keywords： control strategies / feeding / immunity / insect vector / microorganism / plant virus

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Intelligent diagnosis methods for plant machinery

Huaqing WANG, Peng CHEN, Shuming WANG,

Frontiers of Mechanical Engineering 2010, Volume 5, Issue 1, Pages 118-124 doi: 10.1007/s11465-009-0084-z

Abstract: several intelligent diagnostic approaches based on artificial neural network and fuzzy algorithm for plantdiagnostic methods have been successfully applied to condition diagnosis in different types of practical plant

Keywords： intelligent diagnosis neural network fuzzy algorithm adaptive filtering plant machinery

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Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 2, Pages 102-112 doi: 10.15302/J-FASE-2016100

Abstract: involved in these two processes have been identified, many aspects of the molecular mechanisms of the planttechniques (e.g., genomics, proteomics and transcriptomics) has provided a great opportunity to explore plant–pathogenThen, we focus on reviewing the progress in genome-wide PPI networks related to plant–pathogen interactions, including pathogen-centric PPI networks, plant-centric PPI networks and interspecies PPI networks betweenWe anticipate genome-wide PPI network analysis will provide a clearer understanding of plant–pathogen

Keywords： plant–pathogen interactions systems biology omics plant immunity protein–protein interaction network

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Causations of failure in megaprojects: A case study of the Ajaokuta Steel Plant project

Oluwole Alfred OLATUNJI

Frontiers of Engineering Management 2018, Volume 5, Issue 3, Pages 334-346 doi: 10.15302/J-FEM-2018011

Abstract: uses project organizational theories to draw lessons from a historic megaproject, the Ajaokuta Steel PlantDespite about 1400% overrun in cost, the success achieved on the plant was 28% at commissioning.

Keywords： industrial projects megaprojects Nigeria project organizational theories steel plant

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Rhizosphere immunity: targeting the underground for sustainable plant health management

Zhong WEI, Ville-Petri FRIMAN, Thomas POMMIER, Stefan GEISEN, Alexandre JOUSSET, Qirong SHEN

Frontiers of Agricultural Science and Engineering 2020, Volume 7, Issue 3, Pages 317-328 doi: 10.15302/J-FASE-2020346

Abstract:

Managing plant health is a great challenge for modern food production and is further complicated byHere we present the concept of rhizosphere immunity, in which plant health is considered as an ecosystemThese interactions can potentially extend the innate plant immune system to a point where the rhizosphereWe suggest that considering plant health from a meta-organism perspective will help in developing multidisciplinarypathogen management strategies that focus on steering the whole plant-microbe-soil networks instead

Keywords： rhizosphere soil microbiome plant immunity microbial ecology plant health soilborne pathogens

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Title Author Date Type Operation

regions included in a bicistronic vector enhances and stabilizes follistatin gene expressions in both transgeniccells and transgenic mice

Xiaoming HU,Jing GUO,Chunling BAI,Zhuying WEI,Li GAO,Tingmao HU,Shorgan BOU,Guangpeng LI

Journal Article

Functional characterization of caffeic acid

Fengyan WU, Zhenying WU, Aiguo YANG, Shanshan JIANG, Zeng-Yu WANG, Chunxiang FU

Journal Article

Advances of Transgenic Breeding on Rapeseed (Brassica napus)

Guan Chunyun

Journal Article

Enlighenments for China from the Industrialization of the Transgenic Atlantic Salmon in the US

Hu Wei and Zhu Zuoyan

Journal Article

Construction of multiple shRNA vectors targeting PEDV and TGEV and production of transgenic SCNT porcine

Jianwen CHEN, Kaiyuan PAN, Zhen CHEN, Biao DING, Dandan SONG, Wenbin BAO, Yunhai ZHANG

Journal Article

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Journal Article

ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG

Journal Article

conventional mature B cells and compromised specific antibody response in bovine immunoglobulin μ heavy-chain transgenic

Min ZHANG,Xueqian CHENG,Dan CHU,Jingwen LIANG,Yi SUN,Li MA,Beilei XU,Min ZHENG,Meili WANG,Liming REN,Xiaoxiang HU,Qingyong MENG,Ran ZHANG,Ying GUO,Yunping DAI,Robert AITKEN,Ning LI,Yaofeng ZHAO

Journal Article

An introduction to the medicinal plant genome project

Shilin Chen, Li Xiang, Xu Guo, Qiushi Li

Journal Article

HEMIPTERAN-TRANSMITTED PLANT VIRUSES: RESEARCH PROGRESS AND CONTROL STRATEGIES

Journal Article

Intelligent diagnosis methods for plant machinery

Huaqing WANG, Peng CHEN, Shuming WANG,

Journal Article

Systems understanding of plant–pathogen interactions through genome-wide protein–protein interaction

Hong LI,Ziding ZHANG

Journal Article